بسم الله الرحمن الرحيم
((عَنْ أَبِي هُرَيْرَةَ رَضِي اللَّهم عَنْهم أَنَّ رَسُولَ اللَّهِ صَلَّى اللَّهم عَلَيْهِ وَسَلَّمَ قَالَ لَوْلَا أَنْ أَشُقَّ عَلَى أُمَّتِي أَوْ عَلَى النَّاسِ لَأَمَرْتُهُمْ بِالسِّوَاكِ مَعَ كُلِّ صَلَاةٍ ))
رواه البخارى
The antimicrobial effect of aquatic extract of
Salvadora persica on Mycobacterium bovis in vitro
Mehdi Fallah a
, Fatemeh Fallah b
, Mohammad Kamalinejad a
, Mohammad Ali Malekan b
,
Zahrasadat Akhlaghi b
, Mozhgan Esmaeili c,
*
a Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
b Pediatric Infection Research Center, Mofid Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
c Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, International Branch, Tehran, Iran
ARTICLE INFO
Article history:
Received 21 October 2014
Accepted 26 October 2014
Available online 22 December 2014
Keyword:
Mycobacteria
Salvadora persica
Aquatic extract
ABSTRACT
Aim and objective: Infecting around one-third of the world population, Mycobacterium
tuberculosis (MTB) is a serious health-threatening pathogen worldwide. Although TB has
been a well-known disease since ancient times and despite the advances in medical
sciences, large numbers of patients still died because of TB infection.
In 2012, 1.1 million people died as a result of that infection. The development of new
drugs is critical for the future control of tuberculosis (TB), and a number of promising
compounds are currently in the pipeline at various stages of drug discovery and clinical
development. Synthetic antibiotics for MTB treatment brought about the emergence of
multidrug resistant (MDR) and extensively drug-resistant (XTB). MTB strains that has
proved to be a serious challenge to global health; additionally, the long duration of treatment
has various side effects. Therefore, the use of herbal medicines as an alternative or
compliment to synthetic medicines has a considerable importance.
The Miswak plant, which is known as ‘‘Salvadora persica’’ of the ‘‘Salvadoraceae’’ family,
is traditionally used to ensure oral hygiene among Muslim people in developing countries
where it is growing. The antibacterial properties of S. persica originating from various
geographic areas have already been reported.
The aim of this study is to detect the aquatic extract of S. persica activity on Mycobacterium
bovis.
Material and method: Extraction: 100 ml of boiling water was poured on the stem of this plant,
then left at room temperature for 4 h, and then filtered. The crystals were put in a bath for
8 h to get the condensed extract.
Phenotyping: The measurements of synthesized mycolic acids in Middlebrook culture
showed growth of the bacteria. Therefore, even if the antigenic structures were destroyed,
the cell wall did not form; hence the colony growth will be inhibited.
Micro dilution assay: Using the lyophilization powder of the M. bovis which was provided
by the Pasteur Institute, antimicrobial extract susceptibility tests were performed by broth
micro-dilution methods.
Result: The growth of each sample was examined three times with the following
concentrations: 0.5, 1, 2.5, 5, 10, 15, 20, 25, and 30 mg/ml. The minimum inhibitory concentration
for each bacteria sample became positive at 10 mg/ml each t
يقول البحث //
تأثير خلاصه السواك على ميكروب السل يمنع نمو جدار خليه ميكروب السل ويمنع تكاثرها مما يؤدى الى القضاء على ميكروب السل
اللهم صلى على سيدنا محمد ما دام الليل والنهار
